This study aimed to evaluate the postactivation potentiation (PAP) effects of an eccentric overload (EOL) exercise on vertical and horizontal jumps and change of direction (COD) performance. Twelve healthy physically active male subjects were involved in a crossover study. The subjects performed 3 sets of 6 repetitions of EOL half squats for maximal power using a flywheel ergometer. Postactivation potentiation using an EOL exercise was compared between a medium (M-EOL) vs. high inertia (H-EOL) experimental condition. Long jump (LJ) was recorded at 30 seconds, 3, and 6 minutes after both EOL exercises and compared with baseline values (control). The same procedure was used to assess countermovement jump (CMJ) height and peak power and 5-m COD test (COD-5m). A fully Bayesian statistical approach to provide probabilistic statements was used in this study. Long jump performance reported improvements after M-EOL and H-EOL exercise (Bayes factor [BF10] = 32.7, strong; BF10 = 9.2, moderate), respectively. Countermovement jump height (BF10 = 135.6, extreme; BF10 > 200, extreme), CMJ peak power (BF10 > 200, extreme; BF10 = 56.1, very strong), and COD-5m (BF10 = 55.7, very strong; BF10 = 16.4, strong) reported improvements after M-EOL and H-EOL exercise, respectively. Between analysis did not report meaningful differences in performance between M-EOL and H-EOL exercises. The present outcomes highlight that PAP using an EOL (M-EOL and H-EOL) improves LJ, CMJ height, CMJ peak power, and COD-5m in male athletes. The optimal time window for the PAP effect was found for both EOL conditions from 3 to 6 minutes. However, M-EOL and H-EOL produce similar PAP effect on LJ, CMJ, and COD-5m tasks.